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Antibiotic Selection

Antibiotic Selection

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...

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Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...

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Updated: Jun 11, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

[Interpretive reading of enterobacteria antibiograms].

Ferran Navarro¹, Elisenda Miró, Beatriz Mirelis

¹Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, España; Universitat Autònoma de Barcelona, Barcelona, España. fnavarror@santpau.cat

Enfermedades Infecciosas Y Microbiologia Clinica

|June 29, 2010

Summary

Understanding antimicrobial resistance patterns is key. Bacterial resistance to antibiotics like beta-lactams, aminoglycosides, and quinolones arises from natural traits and acquired mechanisms, including enzymes and mutations.

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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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Published on: March 3, 2023

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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Area of Science:

Microbiology
Molecular Biology
Genetics

Context:

Antimicrobial resistance (AMR) is a growing global health threat.
Understanding the genetic basis of bacterial resistance is crucial for effective treatment.
Antibiograms provide a snapshot of bacterial susceptibility to antibiotics.

Purpose:

To elucidate the mechanisms underlying bacterial resistance patterns.
To differentiate between intrinsic and acquired resistance mechanisms.
To correlate specific resistance phenotypes with underlying genetic determinants.

Summary:

Bacterial resistance patterns are a combination of inherent species-specific traits and acquired resistance.
In Enterobacteriaceae, resistance to beta-lactams and aminoglycosides is primarily mediated by specific inactivating enzymes.
Quinolone resistance often results from sequential chromosomal mutations, with recent evidence implicating plasmid-mediated genes.

Impact:

Informing the development of novel antimicrobial strategies.
Guiding the interpretation of antibiograms for clinical decision-making.
Enhancing our understanding of AMR evolution and dissemination.