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Related Experiment Videos

Complete nucleotide sequence of SV40 DNA.

W Fiers, R Contreras, G Haegemann

    Nature
    |May 11, 1978
    PubMed
    Summary
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    The complete DNA sequence of the simian virus 40 (SV40) genome reveals gene locations and novel coding strategies. This analysis details the non-random codon usage and complex splicing of viral messenger RNAs.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genomics

    Background:

    • Simian virus 40 (SV40) is a well-characterized DNA virus.
    • Understanding viral genome organization is crucial for molecular virology.
    • Previous knowledge of SV40 genes existed, but a complete sequence provided a framework for precise mapping.

    Purpose of the Study:

    • To determine the complete 5,224 base-pair DNA sequence of the SV40 genome.
    • To precisely locate known genes on the SV40 genome.
    • To analyze coding strategies, including gene overlap, mRNA splicing, and codon usage.

    Main Methods:

    • DNA sequencing of the entire SV40 genome.
    • Bioinformatic analysis to identify gene locations and open reading frames.
    • Deduction of amino acid sequences from nucleotide sequences.

    Related Experiment Videos

  • Analysis of codon usage patterns.

    • Main Results:

    • The complete 5,224 bp DNA sequence of SV40 was determined.
    • Precise locations of known viral genes were established.
    • The T antigen is encoded by non-contiguous genomic regions, requiring mRNA splicing.
    • The VP1 gene overlaps with VP2 and VP3 genes in the late region, read in a different frame.
    • Non-random, but similar, codon usage was observed in both early and late regions, with absence of specific codon types (NUC, NCG, CGN).

    Conclusions:

    • The complete SV40 DNA sequence provides a definitive map of its genome.
    • SV40 employs complex gene expression strategies, including mRNA splicing and overlapping genes.
    • The deduced amino acid sequences offer insights into viral protein structure and function.
    • Non-random codon usage suggests evolutionary selection pressures on viral gene expression.