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Related Concept Videos

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Related Experiment Video

Updated: Jun 11, 2026

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

The development of peripheral cold neural circuits based on TRPM8 expression.

Y Takashima1, L Ma, D D McKemy

  • 1Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 90089, USA.

Neuroscience
|June 29, 2010
PubMed
Summary

The cold and menthol receptor TRPM8

Area of Science:

  • Neuroscience
  • Sensory Biology
  • Developmental Biology

Background:

  • Afferent nerve fibers detect environmental stimuli via TRP channels.
  • TRPM8 detects cold across a range of temperatures.
  • TRPM8 neuron diversity suggests varied functions.

Purpose of the Study:

  • To investigate the developmental timeline of TRPM8 expression.
  • To understand the establishment of functional cold circuits.
  • To examine neurochemical phenotype transitions in TRPM8 neurons.

Main Methods:

  • Utilized a Trpm8(GFP) transgenic mouse line.
  • Tracked GFP expression from embryonic day 15.5.
  • Assessed anatomical localization and neural activity.
  • Monitored neurochemical marker expression (TRPV1, CGRP, peripherin, NF200).

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A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
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Published on: March 17, 2015

Novel Assay for Cold Nociception in Drosophila Larvae
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Novel Assay for Cold Nociception in Drosophila Larvae

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Main Results:

  • Trpm8(GFP) expression initiated before E15.5, reaching adult levels later.
  • Central axons reached the spinal cord dorsal horn by E18.5.
  • Functional cold circuits matured after the first postnatal week.
  • Neurochemical phenotype transitioned, with adult markers present by postnatal day 14.

Conclusions:

  • Active cold neural circuits are fully established by the second postnatal week.
  • Development involves significant extrinsic or intrinsic mechanisms prior to this stage.
  • TRPM8 neuron development is a complex process influencing sensory perception.