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Updated: Jun 11, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Single-molecule spectroscopy using microfluidic platforms.

Samuel Kim1, Richard N Zare

  • 1Department of Chemistry, Stanford University, Stanford, California, USA.

Methods in Enzymology
|June 29, 2010
PubMed
Summary
This summary is machine-generated.

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This study introduces a microfluidic chip for single-molecule analysis using capillary electrophoresis (CE). The system captures and analyzes separated biomolecules, enabling identification of molecular clusters in complex mixtures.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidics offers precise control for single-molecule experiments.
  • Capillary electrophoresis (CE) on microchips separates species by charge and size, but identification is challenging for complex mixtures.
  • Sticky biomolecules in complex mixtures require advanced separation and identification techniques.

Purpose of the Study:

  • To develop a microfluidic system for high-resolution separation and identification of biomolecules.
  • To enable single-molecule analysis of complex biological samples.
  • To identify biomolecular clusters using fluorescence and correlation spectroscopy.

Main Methods:

  • Utilizing a surfactant mixture system for capillary electrophoresis (CE) on a poly(dimethylsiloxane) (PDMS) microchip.

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

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Last Updated: Jun 11, 2026

Fluorescence detection methods for microfluidic droplet platforms
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Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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  • Employing microvalves to capture separated peaks within a 50-pL chamber.
  • Analyzing fluorescence signals with correlation spectroscopy to determine molecular diffusion characteristics.

    • Main Results:

    • Successful separation and capture of chemical species on a microchip.
    • Extraction of molecular diffusion characteristics from fluorescence signals.
    • Identification of biomolecular clusters within a model immunocomplex system.

    Conclusions:

    • The developed microfluidic CE system effectively separates and identifies biomolecular clusters.
    • This platform provides a novel approach for single-molecule analysis of complex biological mixtures.
    • The integration of microfluidics, CE, and correlation spectroscopy enhances molecular identification capabilities.