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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Single mRNA tracking in live cells.

Hye Yoon Park¹, Adina R Buxbaum, Robert H Singer

¹Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, USA.

Methods in Enzymology

|June 29, 2010

Summary

This summary is machine-generated.

mRNA localization ensures proteins are at the right place in cells. New techniques allow real-time tracking of single mRNA molecules, revealing insights into RNA transport dynamics.

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Last Updated: Jun 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

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Published on: June 4, 2019

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

Area of Science:

Cell Biology
Molecular Biology
Biophysics

Background:

Asymmetric mRNA distribution is common across cell types.
mRNA localization directs protein function to specific cellular sites.
Posttranscriptional mRNA movement is a key regulatory mechanism.

Purpose of the Study:

To provide an overview of single particle tracking approaches.
To describe methodologies for detecting single mRNA molecules.
To highlight advances in visualizing mRNA dynamics in living cells.

Main Methods:

Single particle tracking (SPT) techniques.
Real-time imaging of single mRNA molecules.
Methodologies for mRNA detection and quantification.

Main Results:

SPT enables detailed analysis of mRNA motion.
Real-time visualization reveals complex RNA transport systems.
Advanced detection methods improve understanding of mRNA localization.

Conclusions:

Single mRNA tracking offers powerful insights into cellular RNA dynamics.
Technical advancements are crucial for studying mRNA transport.
Understanding mRNA localization is vital for cell function.