Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Bone Cells and Tissue01:30

Bone Cells and Tissue

Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the periosteum and...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct probabilistic quantification of mosaic loss of chromosome Y from sequencing data.

bioRxiv : the preprint server for biology·2026
Same author

Myeloid Cell Function and Cytokine Profiles in Paediatric Haemophilia A: Insights From FVIII and Emicizumab Prophylaxis.

Journal of cellular and molecular medicine·2026
Same author

Implantable vascularized endocrine constructs for clinically scalable insulin delivery.

Trends in biotechnology·2026
Same author

Highlighting the contributions of Professor T. Ray Bradley to ISEH and the field of Experimental Hematology.

Experimental hematology·2026
Same author

Disruption of CSF-1 receptor-mediated metal ion homeostasis in the murine brain promotes neurodegenerative disease.

The Journal of clinical investigation·2026
Same author

Septins buffer actomyosin forces to protect the nucleus from genotoxic mechanical stress.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 11, 2026

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
11:47

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Dendritic cell-mediated in vivo bone resorption.

Radhashree Maitra1, Antonia Follenzi, Arash Yaghoobian

  • 1Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|June 29, 2010
PubMed
Summary

Dendritic cells (DCs), a type of immune cell, can form multinucleated giant cells that resorb bone. This finding suggests DCs may play a role in bone loss during inflammatory diseases.

More Related Videos

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

Related Experiment Videos

Last Updated: Jun 11, 2026

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
11:47

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

Area of Science:

  • Immunology
  • Bone Biology
  • Cell Biology

Background:

  • Osteoclasts are key cells in bone remodeling, derived from monocyte/macrophage precursors.
  • Inflammatory conditions can trigger new osteoclast formation.
  • The potential for other cell types to form bone-resorbing giant cells is largely unexplored.

Purpose of the Study:

  • To investigate if dendritic cells (DCs) can form multinucleated giant cells with bone-resorbing activity.
  • To analyze the role of DCs in aseptic osteolysis using an animal model.

Main Methods:

  • Utilized an animal model of calvaria-induced aseptic osteolysis.
  • Employed FACS analysis and confocal microscopy to track GFP-labeled immature DCs.
  • Used chromosomal painting to identify DC nuclei within giant cells.
  • Observed DC recruitment and activity in Csf1r(-/-) mice with depleted resident osteoclasts.

Main Results:

  • Injected immature DCs were recruited to osteolysis sites and found in bone-resorbing pits, expressing cathepsin K.
  • Female DC nuclei were identified within giant cells in male mice at osteolysis sites.
  • Conventional DCs induced osteolysis in Csf1r(-/-) mice, even with limited resident osteoclasts and macrophages.

Conclusions:

  • Dendritic cells can differentiate into multinucleated giant cells capable of bone resorption.
  • DCs may contribute significantly to bone resorption in inflammatory bone diseases.
  • This study reveals a novel function for DCs in bone remodeling.