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Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: Jun 11, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

What happens when replication and transcription complexes collide?

Richard T Pomerantz1, Mike O'Donnell

  • 1The Rockefeller University, Howard Hughes Medical Institute, New York, NY, USA.

Cell Cycle (Georgetown, Tex.)
|June 29, 2010
PubMed
Summary
This summary is machine-generated.

Replication forks colliding with transcription complexes can cause genomic instability. Studies in E. coli reveal mechanisms, including auxiliary helicases, that help the bacterial replisome navigate these roadblocks during DNA replication.

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Related Experiment Videos

Last Updated: Jun 11, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Replication fork arrest caused by transcription-replication collisions is a major source of genomic instability and cell death.
  • Understanding how cells manage these conflicts is crucial for maintaining genome integrity.

Purpose of the Study:

  • To review current knowledge on how bacterial replication machinery resolves collisions with transcription complexes.
  • To highlight recent in vitro and in vivo findings regarding these interactions.

Main Methods:

  • Review of recent in vitro studies on E. coli replisome-RNAP polymerase (RNAP) collisions.
  • Analysis of in vivo and in vitro data supporting the role of auxiliary DNA helicases.

Main Results:

  • Identified novel mechanisms for resolving replisome-RNAP polymerase (RNAP) encounters in vitro.
  • Provided evidence supporting the role of auxiliary helicases in overcoming transcription roadblocks during replication.

Conclusions:

  • Bacterial replisomes possess mechanisms to navigate transcription complexes, preventing genomic instability.
  • Auxiliary DNA helicases are key players in facilitating replication fork progression past transcription roadblocks.