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Updated: Jun 11, 2026
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: December 22, 2020
Simultaneous intracellular chloride and pH measurements using a GFP-based sensor
Daniele Arosio1, Fernanda Ricci, Laura Marchetti
1National Enterprise for nanoScience and nanoTechnology, Istituto Nanoscienze, Consiglio Nazionale delle Ricerche and Scuola Normale Superiore, Pisa, Italy. daniele.arosio@cnr.it
Abstract:
Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.
