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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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Related Experiment Video

Updated: Jun 11, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Interplay between Cdh1 and JNK activity during the cell cycle.

Gustavo J Gutierrez1, Toshiya Tsuji, Meifan Chen

  • 1Signal Transduction Program, Sanford-Burnham Medical Research Institute, La Jolla, California 92037, USA.

Nature Cell Biology
|June 29, 2010
PubMed
Summary

The anaphase-promoting complex/cyclosome (APC/C) targets the stress-activated kinase JNK for degradation during cell cycle exit. JNK directly phosphorylates APC/C(Cdh1), impacting its function and revealing a novel cell cycle role for JNK.

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) with its coactivator Cdh1 regulates cell cycle progression through targeted protein degradation.
  • Key cell cycle regulators are degraded by APC/C(Cdh1) to ensure proper cell division and genomic stability.

Purpose of the Study:

  • To investigate the role of the stress-activated kinase JNK in cell cycle regulation.
  • To determine if JNK is a substrate of the APC/C(Cdh1) complex.
  • To elucidate the regulatory interaction between JNK and APC/C(Cdh1) during cell cycle progression.

Main Methods:

  • Western blotting to detect protein levels.
  • Immunofluorescence microscopy to assess protein localization and cell cycle progression.
  • In vitro kinase assays to study phosphorylation events.

Main Results:

  • Nuclear JNK is degraded by APC/C(Cdh1) during mitotic exit and G1 phase.
  • Expression of non-degradable JNK leads to prometaphase arrest and abnormal mitotic spindles.
  • JNK directly phosphorylates Cdh1 during G2/M phase, altering Cdh1 localization and APC/C activity.

Conclusions:

  • JNK is a novel substrate of APC/C(Cdh1), linking stress signaling to cell cycle control.
  • JNK phosphorylation of Cdh1 provides a negative feedback mechanism to regulate APC/C activity during G2/M.
  • This interaction uncovers a critical function for JNK in coordinating cell cycle progression and mitotic fidelity.