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Published on: February 17, 2019
[PKC isoform selectivity and radiation-induced apoptosis of HepG2 cells]
Qiong Xia1, Chuan-gang Li, Ai-min Sun
1Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xiaqiong@fimmu.com
Objective:
To investigate the expressions of protein kinase C (PKC) isoforms in X-ray-exposed HepG2 cells and identify the PKC isoforms that induce radioresistance in HepG2 cells.
Methods:
Cultured HepG2 cells were divided into control group and 6 Gy radiation group for corresponding treatments. The fluorescence intensity (FI) and the percentage of positive cells were determined using flow cytometry.
Results:
The FI of PKCalpha and PKCdelta were 2.28 and 5.05 in the radiation group, respectively, significantly higher than those in the control group (P<0.05). The percentages of PKCalpha- and PKCdelta -positive cells were significantly higher in the radiation group than in the control group (P<0.05). The FI and the percentages of PKC zeta, gamma, epsilon, zeta positive cells were rather low and showed no significant differences between the two groups (P>0.05); PKCbeta expression was not detected in the two groups of cells. The apoptosis rates of the control and radiation groups were 1.73% and 20.90%, respectively.
Conclusion:
PKCalpha and PKCdelta may be involved in protecting HepG2 cells from radiation-induced apoptosis.
