Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Host Species Contribution to the Spatiotemporal Dynamics of the 2024-2025 H5N1 Epidemic in Italy.

Influenza and other respiratory viruses·2026
Same author

Emergence and Rapid Spread of a New Reassortant High Pathogenicity H5N1 Clade 2.3.4.4b Avian Influenza Virus in Nigeria.

Influenza and other respiratory viruses·2026
Same author

A Newcastle disease outbreak in backyard and free-range poultry in Slovenia.

Frontiers in veterinary science·2026
Same author

Metagenomic sequencing of zoonotic viruses: evaluation of a CRISPR-Cas-based rRNA depletion system.

Veterinaria italiana·2026
Same author

A multi-disciplinary approach to identify spillover interfaces of bat coronaviruses to pig farms in Italy.

PloS one·2025
Same author

Origin and Fate of Micronuclei on the Road to Chromoanagenesis.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Jun 11, 2026

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Replication dynamics at common fragile site FRA6E.

Elisa Palumbo1, Laura Matricardi, Elena Tosoni

  • 1Department of Biology, University of Padova, Via U. Bassi 58/b, 35131, Padova, Italy.

Chromosoma
|June 30, 2010
PubMed
Summary

Replication at common fragile site FRA6E is not inherently impaired, but chromosome breakages occur at early/late replication zones. Aphidicolin may exacerbate breakage by extending replication timing differences.

More Related Videos

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jun 11, 2026

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Genetics
  • Molecular Biology
  • Cell Biology

Background:

  • Common fragile sites (CFS) are genomic regions prone to breakage.
  • FRA6E is a frequently expressed CFS harboring the PARK2 gene.
  • Understanding replication dynamics at CFS is crucial for genomic stability.

Purpose of the Study:

  • To investigate the replication dynamics at the common fragile site FRA6E.
  • To determine if replication parameters differ at FRA6E compared to other genomic loci.
  • To assess the impact of aphidicolin-induced stress on FRA6E replication.

Main Methods:

  • Molecular combing to analyze replication fork progression.
  • Interphase fluorescent in situ hybridisation (FISH) to determine replication timing.
  • Culturing primary human lymphocytes under normal and aphidicolin-treated conditions.

Main Results:

  • FRA6E exhibits heterogeneous, mid-late replication timing.
  • Molecular combing showed no specific replication abnormalities at FRA6E.
  • PARK2 gene locus is situated within an early/late replication transition zone.
  • Aphidicolin treatment resulted in slow, unidirectional forks but no specific FRA6E response.

Conclusions:

  • Replication itself is not impaired at FRA6E, but breakages occur at replication transition zones.
  • Aphidicolin may increase FRA6E instability by prolonging the replication timing gap.
  • Findings offer insights into fragile site instability mechanisms.