Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protected fish spawning aggregations as self-replenishing reservoirs for regional recovery.

Proceedings. Biological sciences·2023
Same author

The Sea Spray Chemistry and Particle Evolution study (SeaSCAPE): overview and experimental methods.

Environmental science. Processes & impacts·2022
Same author

Plankton reconstruction through robust statistical optical tomography.

Journal of the Optical Society of America. A, Optics, image science, and vision·2021
Same author

Underwater dual-magnification imaging for automated lake plankton monitoring.

Water research·2021
Same author

Self-localization of a mobile swarm using noise correlations with local sources of opportunity.

The Journal of the Acoustical Society of America·2018
Same author

A swarm of autonomous miniature underwater robot drifters for exploring submesoscale ocean dynamics.

Nature communications·2017

Related Experiment Video

Updated: Jun 11, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Enhanced extended range underwater imaging via structured illumination.

Jules S Jaffe1

  • 1Marine Physical Lab, Scripps Institution of Oceanography, University of California San Diego, La Jolla, 92093-0238,USA. jules@mpl.ucsd.edu

Optics Express
|July 1, 2010
PubMed
Summary

Structured illumination enhances underwater imaging contrast and range by projecting light in a grid pattern. This method offers faster image collection than single-beam systems, with performance gains increasing with beam spacing.

More Related Videos

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging
09:19

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging

Published on: April 18, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jun 11, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging
09:19

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging

Published on: April 18, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

  • Optical Engineering
  • Underwater Imaging Systems

Background:

  • Underwater imaging systems face challenges with contrast and range limitations.
  • Conventional systems often use broad light projection (sheet or area).

Purpose of the Study:

  • To explore structured illumination for enhancing underwater imaging contrast and range.
  • To compare structured illumination with conventional methods via simulations.

Main Methods:

  • Transmitting light in a time-sequenced grid pattern (1D or 2D).
  • Using a receiver with matching grid sensitivity and superimposing time-sequenced images.
  • Simulating performance in challenging underwater environments.

Main Results:

  • Structured illumination offers performance gains over conventional systems.
  • Gains in contrast and resolution are achievable, particularly with larger beam spacing.
  • Faster image acquisition is possible compared to single-beam scanning.

Conclusions:

  • Structured illumination is a viable technique for improving underwater imaging.
  • Trade-offs exist between speed, contrast, and resolution based on beam spacing.
  • The method presents a parallel implementation of laser line scan systems.