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Related Experiment Video

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Full-range polarization-sensitive swept-source optical coherence tomography by simultaneous transversal and spectral

Masahiro Yamanari1, Shuichi Makita, Yiheng Lim

  • 1Computational Optics Group in the University of Tsukuba, Tsukuba, Ibaraki, Japan. yamanari@optlab2.bk.tsukuba.ac.jp

Optics Express
|July 1, 2010
PubMed
Summary

Polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) now offers full-range imaging of birefringent tissues. This advancement overcomes previous axial range limitations for enhanced in vivo visualization.

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Area of Science:

  • Biomedical Optics
  • Ophthalmic Imaging
  • Medical Imaging Technology

Background:

  • Polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) enables in vivo 3D phase-retardation imaging of birefringent biological tissues.
  • Standard PS-SS-OCT uses continuous source polarization modulation, offering high speed but limited axial measurement range.
  • Complex conjugate ambiguity is a challenge in achieving full-range imaging with PS-SS-OCT.

Purpose of the Study:

  • To enhance the axial measurement range of PS-SS-OCT while maintaining polarization-sensitive imaging capabilities.
  • To combine polarization modulation with the B-M-mode scan (BM-scan) method for full-range imaging.
  • To demonstrate the system's effectiveness in visualizing birefringent structures within the retina.

Main Methods:

  • Implemented a B-M-mode scan (BM-scan) method with transversal phase modulation to resolve complex conjugate ambiguity.
  • Combined BM-scan with continuous source polarization modulation, utilizing different scanning directions for optimal performance.
  • Employed numerical cancellation of phase fluctuations before demodulating B-scan modulation.

Main Results:

  • Achieved an extended axial measurement range of 5.35 mm after removing complex conjugate artifacts.
  • Obtained a high signal-to-conjugate ratio of 40.5 dB.
  • Demonstrated high-resolution (11.4 microm) in vivo retinal imaging, visualizing the birefringence of fibrous tissues like the retinal nerve fiber layer, sclera, and lamina cribrosa.

Conclusions:

  • The combined PS-SS-OCT and BM-scan method successfully overcomes the axial range limitation of traditional PS-SS-OCT.
  • This technique provides full-range, polarization-sensitive imaging, crucial for detailed analysis of birefringent biological tissues.
  • The system is effective for in vivo ophthalmic imaging, offering valuable insights into the microstructure of the retina and surrounding tissues.