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Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Related Experiment Video

Updated: Jun 11, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Fiber probe based microfluidic raman spectroscopy.

P C Ashok¹, G P Singh, K M Tan

¹SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, Fife, KY16 9SS, UK.

|July 1, 2010

Summary

This summary is machine-generated.

A new fiber probe Raman detection system on a microfluidic chip offers portable, sensitive analysis. This lab-on-a-chip device enables rapid detection of biological analytes like urea at physiological levels.

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Last Updated: Jun 11, 2026

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The Microfluidic Probe: Operation and Use for Localized Surface Processing

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Area of Science:

Analytical Chemistry
Spectroscopy
Microfluidics

Background:

Raman spectroscopy in microfluidics often suffers from high background noise and limited portability.
Conventional probe designs can be bulky and inflexible, hindering integration into compact systems.

Purpose of the Study:

To develop a novel, portable fiber probe-based Raman detection system integrated into a microfluidic platform.
To enhance sensitivity and reduce background noise for biological analyte detection.

Main Methods:

A split Raman fiber probe was embedded directly into a polydimethylsiloxane (PDMS) microfluidic chip.
The system was designed for alignment-free operation and flexible collection geometry.
Proof-of-concept demonstrated urea detection at physiological levels.

Main Results:

The integrated system demonstrated reduced background noise compared to previous methods.
The device achieved sensitive urea detection at relevant human physiological concentrations.
Low acquisition times were achieved, indicating rapid analysis capabilities.

Conclusions:

The developed system offers a portable and sensitive solution for Raman spectroscopy on a microfluidic platform.
This technology paves the way for lab-on-a-chip devices for biological and environmental sensing.
The alignment-free, embedded probe design enhances usability and performance.