Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Sampling Methods: Overview01:06

Sampling Methods: Overview

A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling. 
In analytical chemistry, the choice of sampling...
Sampling Continuous Time Signal01:11

Sampling Continuous Time Signal

In signal processing, a continuous-time signal can be sampled using an impulse-train sampling technique, followed by the zero-order hold method. Impulse-train sampling involves the use of a periodic impulse train, which consists of a series of delta functions spaced at regular intervals determined by the sampling period. When a continuous-time signal is multiplied by this impulse train, it generates impulses with amplitudes corresponding to the signal's values at the sampling points.
In the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond DOTATATE: [<sup>68</sup>Ga]NODAGA-LM3 PET/CT unmasks the culprit tumor in tumor-induced osteomalacia.

European journal of nuclear medicine and molecular imaging·2026
Same author

Beyond tumor tracing: physiological uptake of sympathetic ganglia on somatostatin receptor PET/CT - prevalence, patterns, and biological rationale.

European journal of nuclear medicine and molecular imaging·2026
Same author

Prognostic Value of Next-Generation Flow Cytometry Versus Dual-Tracer PET/CT for Minimal Residual Disease Assessment in Multiple Myeloma.

Cancer medicine·2026
Same author

Automated anatomically guided quality assessment for OCTA via multi-region analysis and statistical calibration.

Biomedical optics express·2026
Same author

Regional dopaminergic dysfunction patterns discriminate Parkinson's disease from multiple system atrophy parkinsonian subtype.

Clinical parkinsonism & related disorders·2026
Same author

Single-atom substitution redirects KatG reactivity from cofactor biogenesis to stereoselective sulfoxidation.

Nature communications·2026

Related Experiment Video

Updated: Jun 11, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Generic real-time uniform K-space sampling method for high-speed swept-source optical coherence tomography.

Jiefeng Xi1, Li Huo, Jiasong Li

  • 1Department of Biomedical Engineering, Johns Hopkins University, 720 Rutland Avenue, Baltimore, MD 21205, USA.

Optics Express
|July 1, 2010
PubMed
Summary

We created a real-time uniform K-space sampling (Rt-UKSS) method for high-speed swept-source optical coherence tomography (SS-OCT). This innovation simplifies data acquisition and processing for faster, more efficient imaging of biological tissues.

More Related Videos

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Jun 11, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

  • Biomedical Optics
  • Medical Imaging Technology

Background:

  • High-speed swept-source optical coherence tomography (SS-OCT) systems require efficient data acquisition.
  • Current methods often rely on numerical calibration and over-sampling, increasing processing demands.

Purpose of the Study:

  • To develop a universal, real-time uniform K-space sampling (Rt-UKSS) method for SS-OCT.
  • To reduce the need for over-sampling and simplify data processing in SS-OCT.

Main Methods:

  • Generated an external clock synchronized with an interferometric calibration signal for point-by-point data acquisition.
  • Implemented the Rt-UKSS method in a Fourier-domain mode-locked laser (FDML)-based SS-OCT system operating at 40 kHz.

Main Results:

  • Achieved uniform K-space sampling without over-sampling.
  • Demonstrated real-time imaging of biological tissues.
  • Measured an axial resolution of 9.3 µm and a detection sensitivity exceeding 120 dB.

Conclusions:

  • The Rt-UKSS method is adaptive and applicable to various SS-OCT systems.
  • This method significantly reduces demands on digitization, data processing, and storage speed.
  • Enables high-speed, high-resolution, and high-sensitivity imaging in SS-OCT.