Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Integrated Hardware-Software Platform for Automated Thermodynamic Characterization of Gas-Solid Interfaces Using a Resonant Microcantilever.

Micromachines·2026
Same author

Deep Volumetric Super-Resolution Imaging in Thick Biological Specimens With Sparse Scanning SIM.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

WNT5a export onto extracellular vesicles studied at single-molecule and single-vesicle resolution.

The FEBS journal·2025
Same author

Prediction of epileptogenicity in patients with tuberous sclerosis complex using multimodal cerebral MRI.

European journal of radiology·2024
Same author

L-ascorbate Alleviates Chronic Obstructive Pulmonary Disease through the EGF/PI3K/AKT Signaling Axis.

Current medicinal chemistry·2024
Same author

Caveolae disassemble upon membrane lesioning and foster cell survival.

iScience·2024
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Ultra-fast, high-precision image analysis for localization-based super resolution microscopy.

Tingwei Quan1, Pengcheng Li, Fan Long

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, PR China.

Optics Express
|July 1, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a fast image analysis method for super-resolution microscopy, enabling real-time live cell imaging. The technique combines maximum likelihood estimation and Graphics Processing Units (GPUs) for rapid localization and movement detection.

More Related Videos

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Related Experiment Videos

Last Updated: Jun 11, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Area of Science:

  • Biophysics
  • Microscopy
  • Computational Biology

Background:

  • Localization-based super-resolution microscopy offers high-resolution live cell imaging.
  • Slow image analysis speeds currently limit its widespread application.

Purpose of the Study:

  • To develop a rapid image analysis method for super-resolution microscopy.
  • To enable real-time processing for live cell imaging applications.

Main Methods:

  • Implemented a novel image analysis method combining maximum likelihood estimation with Graphics Processing Unit (GPU) acceleration.
  • Tested the method's performance on images from fast EMCCD cameras at full frame rate.

Main Results:

  • The developed method achieves processing speeds suitable for real-time analysis of live cell imaging data.
  • Localization precision and field of view were maintained without compromise.
  • Immediate visualization of cellular movements post-data acquisition was achieved.

Conclusions:

  • This GPU-accelerated maximum likelihood method significantly enhances the speed of super-resolution microscopy image analysis.
  • The technique facilitates immediate insights into dynamic cellular processes, greatly benefiting live cell imaging research.