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Related Experiment Video

Updated: Jun 11, 2026

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
08:35

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Published on: July 19, 2014

In vitro organogenesis using multipotent cells.

Akira Kurisaki1, Yuzuru Ito, Yasuko Onuma

  • 1Organ Development Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.

Human Cell
|July 2, 2010
PubMed
Summary

Developing efficient stem cell differentiation protocols is key for regenerative medicine and drug discovery. This review explores using Xenopus laevis embryos to create organogenesis blueprints and apply them to mammalian stem cell differentiation.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Efficient stem cell differentiation is crucial for regenerative medicine and drug discovery.
  • Existing stem cell populations (ES, somatic, iPS) lack comprehensive differentiation protocols.
  • Organ regeneration in vitro requires precise differentiation blueprints.

Purpose of the Study:

  • To review methods for in vitro organ formation using undifferentiated Xenopus cells.
  • To describe the application of amphibian differentiation protocols to mammalian stem cells.
  • To facilitate the development of efficient in vitro differentiation methodologies.

Main Methods:

  • Utilizing Xenopus laevis embryos as research tools for differentiation protocol development.

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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

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Last Updated: Jun 11, 2026

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
08:35

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Published on: July 19, 2014

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

  • Employing microinjection into embryonic cells to study gene product functions during organogenesis.
  • Reviewing examples of in vitro organ formation methods using Xenopus cells.

    • Main Results:

    • Xenopus embryos offer a robust model for studying organogenesis due to accessibility and developmental similarity to humans.
    • Established amphibian differentiation protocols can be adapted for mammalian stem cells.
    • The study provides examples of in vitro organ formation techniques.

    Conclusions:

    • Xenopus laevis is a valuable model organism for uncovering organogenesis factors and developing differentiation protocols.
    • Amphibian differentiation protocols show promise for advancing mammalian stem cell differentiation.
    • Further research can accelerate the development of efficient in vitro differentiation methods for regenerative medicine.