Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Howler monkey Platy-1 and Alu SINEs: a resource for Alouatta genomics.

Mobile DNA·2026

Same author

Genetic contributions to mitochondrial dysfunction in amyotrophic lateral sclerosis etiology.

HGG advances·2026

Same author

Narrow Versus Broad Phenotype Definitions Affect Genetic Analysis of Language More than Other Broad Autism Phenotype Traits.

Genes·2026

Same author

scTELL: a single-cell ATAC-seq tool for locus-specific transposable element identification in chromatin accessibility.

Mobile DNA·2026

Same author

Genomics Insights Into High-Latitude Adaptation of Tibetan Macaques.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Platy-1 SINEs from Thirteen Diverse Genomes Reveal Callithrichidae Unique Amplification, Recent <i>Alouatta</i> Mobilization and Insights into Platyrrhine Phylogenetics.

Genes·2026

Same journal

Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.

BMC genomics·2026

Same journal

Comparative and population genomics analyses of eared pheasants inhabiting highly varying altitudes.

BMC genomics·2026

Same journal

Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120.

BMC genomics·2026

Same journal

A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.

BMC genomics·2026

Same journal

Multiple genomic approaches reveal geographic structure and local selection signals in invasive Anopheles stephensi from the Horn of Africa and Yemen.

BMC genomics·2026

Same journal

Integration of bulk RNA-seq and scRNA-seq reveals cell subsets and gene signatures associated with Glaesserella parasuis infection.

BMC genomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Mobile element scanning (ME-Scan) by targeted high-throughput sequencing.

David J Witherspoon¹, Jinchuan Xing, Yuhua Zhang

¹Dept. of Human Genetics, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA. david.witherspoon@utah.edu

|July 2, 2010

Summary

This summary is machine-generated.

A new method, Mobile Element Scanning (ME-Scan), efficiently identifies and genotypes mobile element insertions across multiple individuals. This technique overcomes previous limitations in studying mobile element dynamics and evolution in diverse species.

More Related Videos

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Related Experiment Videos

Last Updated: Jun 11, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

Genomics
Molecular Biology
Population Genetics

Background:

Mobile elements (MEs) are crucial genomic components driving mutation and evolution.
Studying ME dynamics is challenging due to the difficulty of identifying rare polymorphic insertions among fixed elements.
Existing methods struggle to genotype numerous ME insertions across many individuals efficiently.

Purpose of the Study:

To develop a novel, efficient method for identifying and genotyping mobile element insertions in whole genomes.
To overcome the limitations of current techniques in studying ME population dynamics and evolution.
To enable high-throughput analysis of ME insertion polymorphisms in multiple individuals.

Main Methods:

Developed Mobile Element Scanning (ME-Scan), a method utilizing ME-specific primers and indexed DNA libraries.
Constructed libraries containing ME insertion junctions and flanking sequences from multiple individuals.
Pooled and sequenced indexed libraries, linking unique junction sequences to individual samples.

Main Results:

Applied ME-Scan to human AluYb8 and AluYb9 subfamilies in four individuals.
Identified 2,758 Alu insertions, including novel and reference genome-matched loci.
Achieved high accuracy with approximately 95% sensitivity and specificity at a sequencing depth of 355,000 reads per sample.

Conclusions:

Mobile Element Scanning (ME-Scan) provides an efficient, sensitive, and specific method for genotyping ME insertions.
The technique is adaptable for various ME families and species, leveraging advancements in high-throughput sequencing.
ME-Scan facilitates large-scale population studies of mobile element dynamics and their evolutionary impact.