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Related Experiment Video

Updated: Jun 11, 2026

In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

Published on: May 11, 2020

Persistent currents and discharge patterns in rat hindlimb motoneurons.

Thomas M Hamm¹, Vladimir V Turkin, Neha K Bandekar

¹Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Division of Neurobiology, 350 W. Thomas Rd., Phoenix, AZ 85013, USA. Thomas.Hamm@chw.edu

Journal of Neurophysiology

|July 2, 2010

Summary

This study measured persistent inward currents (PICs) in rat motoneurons, revealing their role in neuronal firing patterns. These currents influence frequency-current relationships and adaptation, impacting motoneuron discharge characteristics.

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Area of Science:

Neuroscience
Electrophysiology

Background:

Persistent inward currents (PICs) are crucial for motoneuron excitability.
Direct measurements of PICs in rat hindlimb motoneurons are lacking.

Purpose of the Study:

To directly measure PICs in rat hindlimb motoneurons.
To investigate the relationship between PICs and motoneuron discharge characteristics, including frequency-current (f-I) relationships and adaptation.

Main Methods:

Single-electrode somatic voltage clamp in ketamine-xylazine anesthetized rats.
Slow voltage ramps to elicit and measure PICs.
Analysis of current-voltage (I-V) relationships, including negative-slope regions (NSRs).

Main Results:

Most motoneurons (13/19) exhibited PICs with a negative-slope region (NSR).
PICs activated at -52.7 ± 3.89 mV, preceding spike threshold.
PIC amplitudes varied widely and influenced f-I range boundaries and adaptation.

Conclusions:

Direct measurements confirm PICs significantly impact rat motoneuron excitability and firing.
PICs and associated outward currents play a role in frequency adaptation and f-I range.
Findings provide insights into the electrophysiological basis of motoneuron function.