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Isolation of Double Negative αβ T Cells from the Kidney
Published on: May 16, 2014
Changes in lymphocyte function and subsets in dogs with naturally occurring chronic renal failure
Simona Kralova1, Lenka Leva, Miroslav Toman
1Clinic of Dog and Cat Diseases, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. kralovas@post.cz <kralovas@post.cz>
Summary
Chronic renal failure (CRF) in dogs leads to significant immunosuppression, particularly affecting B-cells, Tc-cells, and NK cells. This immune dysfunction in dogs with CRF mirrors that in humans, increasing infection risk.
Area of Science:
- Veterinary Immunology
- Nephrology
- Immunology
Background:
- Chronic renal failure (CRF) is linked to immunosuppression in humans.
- CRF is a potential cause of secondary immunosuppression in dogs.
- Immune system function in dogs with CRF requires further investigation.
Purpose of the Study:
- To evaluate the immunological status of dogs with varying stages of chronic renal failure.
- To identify specific lymphocyte subset changes associated with CRF progression in dogs.
- To compare the immunological findings in dogs with CRF to those in uremic humans.
Main Methods:
- Hematological, biochemical, and immunological blood analyses were conducted.
- Lymphocyte subsets (B-cells, Tc-cells, NK cells) were quantified.
- Lymphocyte proliferation responses to mitogens (concanavalin A, pokeweed) were assessed.
Main Results:
- Lymphopenia increased with the progression of clinical signs in dogs with CRF.
- Significant reductions were observed in B-cells, Tc-cells, and NK cells.
- Dogs with severe CRF and lymphopenia exhibited depressed lymphocyte responses to mitogens.
Conclusions:
- Dogs with CRF experience significant impairment of immune function, including lymphopenia and reduced lymphocyte activity.
- The observed immunological deficits in dogs with CRF are comparable to those in uremic humans.
- Infection is a likely and important complication for dogs suffering from chronic renal failure.
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