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Updated: Jun 11, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
A new cryo-STEM method for imaging food colloids in the scanning electron microscope
V L Gee1, M A Carey, M A E Auty
1Food Processing & Functionality Department, Moorepark Food Research Centre, Teagasc, Fermoy, Co. Cork, Ireland.
Abstract:
A new cryo-scanning transmission electron microscopy (cryo-STEM) technique for imaging casein micelles in a field emission scanning electron microscope is presented. Thin films of micellar casein suspensions on lacey carbon grids were prepared using a modified sample holder developed by Gatan UK. Bright and dark field images were obtained at -135°C showing casein micelles in their frozen hydrated state and in the size range 30-500 nm. Results were compared favorably with published images of casein micelles obtained with conventional cryo-transmission electron microscopy, suggesting that cryo-STEM is a useful alternative technique for visualizing food colloids close to their native state.
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