Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microbial biosensor for trimethylamine using Pseudomonas aminovorans cells.

S Gamati1, J H Luong, A Mulchandani

  • 1Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.

Biosensors & Bioelectronics
|January 1, 1991
PubMed
Summary

A novel biosensor system effectively monitors trimethylamine (TMA) by measuring oxygen uptake differences in microbial electrodes. This method offers a stable and accurate approach for TMA detection in fish extracts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective recognition of xylene isomers using ZnO-SWNTs hybrid gas sensors.

The Analyst·2012
Same author

Amperometric thick-film strip electrodes for monitoring organophosphate nerve agents based on immobilized organophosphorus hydrolase.

Analytical chemistry·2011
Same author

Optimization and characterization of a flow injection electrochemical system for pentachlorophenol assay.

Analytical chemistry·2011
Same author

A Combined Chemical and Electrochemical Approach Using Bis(trifluoroacetoxy)iodobenzene and Glucose Oxidase for the Detection of Chlorinated Phenols.

Analytical chemistry·2011
Same author

Separation and determination of polycyclic aromatic hydrocarbons by solid phase microextraction/cyclodextrin-modified capillary electrophoresis.

Analytical chemistry·2011
Same author

Decontamination of vegetables sprayed with organophosphate pesticides by organophosphorus hydrolase and carboxylesterase (B1).

Applied biochemistry and biotechnology·2007

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Environmental Science

Background:

  • Trimethylamine (TMA) is a key indicator of fish spoilage.
  • Accurate monitoring of TMA is crucial for food quality assessment.
  • Existing analytical methods can be time-consuming and complex.

Purpose of the Study:

  • To develop a sensitive and stable biosensor for trimethylamine (TMA) detection.
  • To utilize microbial electrodes for differential oxygen uptake measurements.
  • To validate the biosensor's performance in analyzing fish tissue extracts.

Main Methods:

  • Development of two microbial electrodes using Pseudomonas aminovorans, one grown on TMA and the other on TMAO.
  • Measurement of differential oxygen uptake in response to varying TMA concentrations.

Related Experiment Videos

  • Optimization of operational and storage conditions for biosensor stability.
  • Comparison of biosensor results with High-Performance Liquid Chromatography (HPLC) for fish tissue samples.

    • Main Results:

    • The biosensor demonstrated a linear response to TMA in the range of 5-26 microM.
    • A minimum detectable level of 2.6 microM TMA was achieved.
    • The biosensor exhibited enhanced stability (up to 20 days) when stored at 4 degrees C overnight.
    • Results from fish tissue extracts showed good agreement with HPLC analysis.

    Conclusions:

    • The developed microbial electrode biosensor system provides a reliable method for TMA monitoring.
    • Optimized storage conditions significantly improve the biosensor's operational lifespan.
    • The biosensor is a viable tool for practical applications in food quality control and analysis.