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High frequency of the IL-2 -330 T/HLA-DRB1*1501 haplotype in patients with multiple sclerosis

Majid Shahbazi1, Danial Roshandel, Hamid Ebadi

  • 1Medical Cellular & Molecular Research Center, Talghani Children Hospital of Golestan University of Medical Sciences, Bolv Janbazan, 4916668197, Gorgan, Iran. shahbazimajid@yahoo.co.uk

Clinical Immunology (Orlando, Fla.)
|July 3, 2010
PubMed

Insights

This study reveals that the HLA-DRB1*1501 allele and the IL-2 -330 T allele significantly increase multiple sclerosis risk. Gene-gene interactions, particularly the HLA-DRB1*1501/T haplotype, are crucial in multiple sclerosis development.

Area of Science:

  • Immunogenetics
  • Neuroimmunology
  • Human Genetics

Background:

  • Multiple sclerosis (MS) is a complex autoimmune disease affecting the central nervous system.
  • Genetic factors, including human leukocyte antigen (HLA) genes and cytokine polymorphisms, are implicated in MS susceptibility.
  • The role of specific alleles and their interactions in MS pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the association of the HLA-DRB1*1501 allele and the Interleukin-2 (IL-2) -330 T/G polymorphism with multiple sclerosis risk.
  • To examine the potential gene-gene interaction between HLA-DRB1*1501 and IL-2 -330 polymorphism in MS susceptibility.

Main Methods:

  • Case-control study involving 360 MS patients and 426 healthy controls.
  • Genotyping of HLA-DRB1*1501 allele and IL-2 -330 T/G polymorphism using SSP-PCR.
  • Statistical analysis using Fisher's exact test and logistic regression for odds ratio (OR) and P-values.

Main Results:

  • The IL-2 -330 T allele was significantly more frequent in MS patients (OR=1.34, P<0.05), with T/T and T/G genotypes also increased.
  • The HLA-DRB1*1501 allele was significantly overrepresented in patients (OR=1.7, P=0.0006).
  • A strong interaction was observed, with the HLA-DRB1*1501/T haplotype showing a markedly higher frequency in patients (OR=16, P<0.0001).

Conclusions:

  • The findings confirm the association of HLA-DRB1*1501 with multiple sclerosis susceptibility.
  • This study highlights the significant role of gene-gene interactions, specifically involving the IL-2 promoter polymorphism and HLA-DRB1*1501, in the development of MS.

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