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Updated: Jun 11, 2026

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
Published on: August 24, 2012
Calcium dynamics in gonadotropin-releasing hormone neurons
Christine L Jasoni1, Nicola Romanò, Stephanie Constantin
1Centre for Neuroendocrinology, Departments of Physiology, University of Otago, Dunedin 9054, New Zealand.
Abstract:
The gonadotropin-releasing hormone (GnRH) neurons represent the key output cells of the neuronal network controlling fertility. Intracellular calcium ion concentration ([Ca(2+)](i)) is likely to be a key signaling tool used by GnRH neurons to regulate and co-ordinate multiple cell processes. This review examines the dynamics and control of [Ca(2+)](i) in GT1 cells, embryonic GnRH neurons in the nasal placode culture, and adult GnRH neurons in the acute brain slice preparation. GnRH neurons at all stages of development display spontaneous [Ca(2+)](i) transients driven, primarily, by their burst firing. However, the intracellular mechanisms generating [Ca(2+)](i) transients, and the control of [Ca(2+)](i) by neurotransmitters, varies markedly across the different developmental stages. The functional roles of [Ca(2+)](i) transients are beginning to be unraveled with one key action being that of regulating the dynamics of GnRH neuron burst firing.
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