Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Machine Learning Prediction of Quality of Life Improvement During Antidepressant Treatment of Patients With Major Depressive Disorder: A STAR*D and CAN-BIND-1 Report.

The Journal of clinical psychiatry·2023

Same author

Cross-disorder GWAS meta-analysis of endocannabinoid DNA variations in major depressive disorder, bipolar disorder, attention deficit hyperactivity disorder, autism spectrum disorder, and schizophrenia.

Psychiatry research·2023

Same author

Influence of <i>CYP2C19</i>, <i>CYP2D6</i>, and <i>ABCB1</i> Gene Variants and Serum Levels of Escitalopram and Aripiprazole on Treatment-Emergent Sexual Dysfunction: A Canadian Biomarker Integration Network in Depression 1 (CAN-BIND 1) Study.

Canadian journal of psychiatry. Revue canadienne de psychiatrie·2023

Same author

The emergence, implementation, and future growth of pharmacogenomics in psychiatry: a narrative review.

Psychological medicine·2023

Same author

Developing an Electroencephalography-Based Model for Predicting Response to Antidepressant Medication.

JAMA network open·2023

Same author

Author Correction: Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.

Nature communications·2023

Same journal

A scalable high-throughput serolomics platform for profiling serum antibody responses in large-scale population-based cohorts.

Nature protocols·2026

Same journal

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026

Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026

Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026

Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026

Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy.

Jens Friedrichs¹, Jonne Helenius, Daniel J Muller

¹Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.

Nature Protocols

|July 3, 2010

Summary

This summary is machine-generated.

This study presents a protocol for atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) to measure HeLa cell adhesion to collagen. This method quantifies cell-extracellular matrix interactions for broader biological insights.

More Related Videos

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

Related Experiment Videos

Last Updated: Jun 11, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Single-cell force spectroscopy (SCFS) quantifies cell adhesion under physiological conditions.
Adhesive interactions are crucial for cell-substrate and cell-cell communication.
Extracellular matrix (ECM) proteins mediate cell adhesion and influence cellular behavior.

Purpose of the Study:

To present a detailed protocol for studying integrin-mediated adhesion of HeLa cells to collagen type I using AFM-based SCFS.
To provide a reproducible method for measuring cell adhesion forces.
To enable quantitative analysis of cell-ECM interactions.

Main Methods:

Functionalization of AFM cantilevers with concanavalin A and supports with collagen type I.
Cell handling and attachment to the AFM cantilever.
Measurement of cell adhesion forces using AFM-based SCFS and subsequent data analysis.

Main Results:

A comprehensive protocol for AFM-based SCFS is described.
The protocol allows for the quantitative measurement of HeLa cell adhesion to collagen.
The method is adaptable for other cell lines and ECM proteins.

Conclusions:

AFM-based SCFS offers a versatile force detection range for studying cell adhesion.
This protocol facilitates the investigation of a broad range of biological questions related to cell-ECM interactions.
The described method can be completed within 2-3 days.