A Novel Approach to Molecular Recognition Surface of Magnetic Nanoparticles Based on Host-Guest Effect

A novel route has been developed to prepared beta-cyclodextrin (beta-CD) functionalized magnetic nanoparticles (MNPs). The MNPs were first modified with monotosyl-poly(ethylene glycol) (PEG) silane and then tosyl units were displaced by amino-beta-CD through the nucleophilic substitution reaction. The monotosyl-PEG silane was synthesized by modifying a PEG diol to form the corresponding monotosyl-PEG, followed by a reaction with 3-isocyanatopropyltriethoxysilane (IPTS). The success of the synthesis of the monotosyl-PEG silane was confirmed with (1)H NMR and Fourier transform infrared (FTIR) spectroscopy. The analysis of FTIR spectroscopy and X-ray photoelectron spectroscopy (XPS) confirmed the immobilization of beta-CD onto MNPs. Transmission electron microscopy (TEM) indicated that the beta-CD functionalized MNPs were mostly present as individual nonclustered units in water. The number of beta-CD molecules immobilized on each MNP was about 240 according to the thermogravimetric analysis (TGA) results. The as-prepared beta-CD functionalized MNPs were used to detect dopamine with the assistance of a magnet.