Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What Is the Effect of Robot Reduction in Displaced Pelvic Fractures? A Multicenter Randomized Clinical Trial.

Clinical orthopaedics and related research·2026
Same author

Individualised progesterone receptor modulator prevention strategies for triple-negative breast cancer in BRCA1 pathogenic variant carriers.

NPJ breast cancer·2026
Same author

Transforming endometrial cancer diagnosis: the case for molecular triage in abnormal uterine and post-menopausal bleeding.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2026
Same author

Mechanism and Therapeutic Potential of Viral Mimicry in Cancer Immunotherapy.

Biomolecules·2026
Same author

Mouse models of immune-related adverse events: progress, challenges, and opportunities.

Trends in cancer·2026
Same author

Cost-Minimization Analysis of the Women's Cancer Risk Identification-Quantitative Polymerase Chain Reaction Test for Endometrial Cancer Epigenetic Biomarker Compared With Transvaginal Ultrasound for Triaging Abnormal Uterine Bleeding.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2026

Related Experiment Video

Updated: Jun 11, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Development and validation of a method for profiling post-translational modification activities using protein

Sonia V Del Rincón1, Jeff Rogers, Martin Widschwendter

  • 1Signal Transduction Program, Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America.

Plos One
|July 3, 2010
PubMed
Summary

We developed a novel protein microarray assay to profile post-translational modifications (PTMs) like ubiquitylation, SUMOylation, and NEDDylation in complex biological samples. This method simplifies PTM analysis for disease research.

More Related Videos

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
10:26

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Published on: November 7, 2019

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Related Experiment Videos

Last Updated: Jun 11, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
10:26

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Published on: November 7, 2019

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Post-translational modifications (PTMs) are crucial for protein function and cellular diversity.
  • Analyzing PTMs proteome-wide is essential for understanding signaling networks but technically challenging.
  • Current methods for large-scale PTM evaluation are limited.

Purpose of the Study:

  • To develop a protein microarray-based assay for profiling PTM activities.
  • To facilitate the analysis of PTMs in complex biological mixtures like cell extracts and pathological specimens.
  • To enable proteome-wide studies of PTMs and their role in biological processes.

Main Methods:

  • Utilized protein microarrays as a substrate for in vitro enzymatic reactions.
  • Applied labeled modifiers (ubiquitin, SUMO1, NEDD8) and ATP regenerating systems.
  • Profiled ubiquitylation, SUMOylation, and NEDDylation using purified ligases and cell/pathological extracts.
  • Validated novel PTM substrates identified through the assay by confirming in vivo modification.

Main Results:

  • Successfully profiled three complex PTMs: ubiquitylation, SUMOylation, and NEDDylation.
  • Demonstrated the assay's capability with both purified enzymes and complex biological extracts.
  • Identified novel PTM substrates, which were further validated in vivo.
  • The assay proved effective across various sample types, including pathological specimens.

Conclusions:

  • The developed protein microarray assay offers advantages in ease of use, speed, scalability, and sample diversity.
  • This methodology allows direct comparison of enzymatic activities in different cell populations or pathological states.
  • Has broad applications for studying PTMs in human diseases and fundamental PTM research.