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Updated: Jun 11, 2026

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

Published on: March 18, 2016

Revealing new mouse epicardial cell markers through transcriptomics.

Lars Bochmann¹, Padmini Sarathchandra, Federica Mori

¹Mouse Biology Unit, European Molecular Biology Laboratory, Monterotondo, Italy. bochmann@embl.it

|July 3, 2010

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Researchers profiled the murine epicardial transcriptome, identifying key genes involved in heart development and repair. This reveals an epicardium-specific response to cardiac injury, offering insights into cardiac regeneration.

Area of Science:

Cardiovascular Biology
Developmental Biology
Regenerative Medicine

Background:

The epicardium is crucial for heart development, contributing to various cardiac cell types and signaling pathways.
Adult epicardium may contain progenitor cells for cardiac repair, but its molecular profile is poorly understood.

Purpose of the Study:

To characterize the murine epicardial transcriptome.
To identify genes involved in epicardial function during development and in response to injury.

Main Methods:

Laser capture microscopy was used to isolate the epicardial layer from adult mouse hearts.
DNA microarrays analyzed the transcription profile before and after myocardial infarction in wildtype and mIGF-1 transgenic mice.

Main Results:

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Last Updated: Jun 11, 2026

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

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In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart

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Published on: April 27, 2016

Epithelial genes like basonuclin and dermokine are enriched in the epicardium.
A subset of differentially expressed genes in the epicardium indicates an injury-specific response after myocardial infarct.

Conclusions:

This study provides the first detailed murine epicardial transcriptome.
The findings offer essential information for understanding epicardial roles in cardiac development and repair.