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Published on: March 11, 2020
Enterobacter spp.: a new evidence causing bacterial wilt on mulberry
Bo Zhu1, GuoFen Wang, GuanLin Xie
1State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China.
Abstract:
Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou, Zhejiang province of China. The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test, electron microscope observation, hypersensitive reaction, Koch's postulates, physiological and biochemical test, biology, fatty acid methyl esters analysis (FAMEs), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), 16s rRNA sequences analysis, and comparative analysis with 7 type strains and 3 reference strains. This is the first report on mulberry disease caused by Enterobacter spp. in the world providing new evidence on induction of the plant disease in this genus. The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.
Insights
This study identifies Enterobacter species as a cause of mulberry wilt disease, a novel finding with implications for disease management and understanding Enterobacter
Area of Science:
- Plant Pathology
- Microbiology
- Bacteriology
Background:
- Mulberry plants are susceptible to various diseases affecting cultivation.
- The specific bacterial agents causing mulberry wilt are not fully understood.
- Enterobacter species are known opportunistic pathogens in humans and environmental microbes.
Purpose of the Study:
- To identify and characterize the bacterial species responsible for mulberry wilt disease.
- To provide the first global report of mulberry disease caused by Enterobacter species.
- To investigate the pathogenic potential of Enterobacter species in plants.
Main Methods:
- Isolation and identification of bacterial strains from wilted mulberry plants.
- Bacteriological tests, electron microscopy, hypersensitive reaction assays, and Koch's postulates.
- Physiological and biochemical tests, fatty acid methyl ester analysis (FAMEs), ERIC-PCR, and 16S rRNA gene sequencing.
Main Results:
- Thirty-six pathogenetic bacterial strains were isolated, with six representative strains identified as Enterobacter species.
- Comprehensive analyses confirmed the role of these Enterobacter strains in inducing mulberry wilt.
- This marks the first documented instance of Enterobacter-induced mulberry disease worldwide.
Conclusions:
- Enterobacter species are identified as causal agents of mulberry wilt disease.
- This discovery is crucial for developing effective mulberry disease management strategies.
- Findings contribute significant scientific knowledge to the study of plant-associated Enterobacter and their pathogenic potential.
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