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Related Experiment Video

Updated: Jun 11, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Multilocus sequence typing for Clostridium difficile.

Ludovic Lemée1, Jean-Louis Pons

  • 1Groupe de Recherche sur les Antimicrobiens et les Micro-organismes, Faculté de Médecine-Pharmacie, Université de Rouen, Rouen Cedex, France.

Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2010
PubMed
Summary

Multilocus sequence typing (MLST) provides a robust method for characterizing Clostridium difficile strains. This approach reveals a clonal population structure and aids in understanding the pathogen's evolution and epidemiology.

Related Experiment Videos

Last Updated: Jun 11, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Area of Science:

  • Microbiology
  • Evolutionary Genetics
  • Epidemiology

Background:

  • Multilocus sequence typing (MLST) is a nucleotide sequence-based method for characterizing allelic polymorphism in housekeeping genes.
  • MLST offers unambiguous sequence data suitable for global sharing in a common web database for bacterial pathogens.

Purpose of the Study:

  • To present the materials, methods, and software required for performing MLST on Clostridium difficile.
  • To establish an MLST scheme for C. difficile using six housekeeping gene loci.
  • To analyze the population structure and evolutionary genetics of C. difficile.

Main Methods:

  • MLST was performed on 74 C. difficile isolates from diverse hosts, geographic origins, and PCR-toxigenic types.
  • Sequence analysis of six housekeeping gene loci was conducted.
  • Linkage disequilibrium and phylogenetic analyses were employed.

Main Results:

  • Thirty-two distinct sequence types (STs) were defined, correlating well with toxigenic types.
  • A clonal population structure was indicated by linkage disequilibrium analysis.
  • Point mutation was found to be the primary driver of evolution, occurring eightfold more frequently than recombination.
  • Phylogenetic analysis revealed no distinct lineages for human versus animal isolates or hypervirulent strains, except for a homogeneous lineage of A-B+ variant isolates.

Conclusions:

  • MLST is a valuable tool for the population and evolutionary genetics of C. difficile.
  • MLST data can serve as typing markers for long-term global epidemiology, potentially combined with virulence gene data.
  • An MLST database for C. difficile is available at the Institut Pasteur Paris.