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Related Concept Videos

Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Jun 11, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

Transposon mutagenesis in Clostridium difficile.

Haitham A Hussain1, Adam P Roberts, Rachael Whalan

  • 1Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2010
PubMed
Summary

Generating random mutations in Clostridium difficile is challenging. This study introduces a new method using the Tn916 transposon for efficient genetic manipulation, creating a valuable insertion library for C. difficile research.

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08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Genetic manipulation of Clostridium difficile is difficult.
  • The conjugative transposon Tn916 is the primary method for random mutagenesis.
  • Tn916 exhibits broad target site insertion in C. difficile.

Purpose of the Study:

  • To develop a reliable method for genetic manipulation of Clostridium difficile.
  • To construct a Tn916 insertion library in the C. difficile 630 strain.

Main Methods:

  • Construction of an erythromycin-sensitive derivative (C. difficile 630Deltaerm).
  • Utilized a Tn916 derivative (Tn916DeltaE) for transposon mutagenesis.
  • Demonstrated multi-site genome insertion of Tn916.

Main Results:

  • Successful generation of an erythromycin-sensitive C. difficile strain.
  • Tn916DeltaE demonstrated insertion at multiple genomic locations.
  • Established a Tn916 insertion library for C. difficile 630.

Conclusions:

  • The developed method enables efficient random mutagenesis in C. difficile.
  • The Tn916 insertion library provides a valuable resource for genetic studies.
  • This approach overcomes previous limitations in C. difficile genetic manipulation.