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A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications
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Published on: January 23, 2021

Constitutive differences between steely and mealy barley samples associated with endosperm modification.

Barbara Ferrari1, Marina Baronchelli, Antonio M Stanca

  • 1C.R.A.-GPG, Genomics Research Centre, Via S. Protaso 302, 29017 Fiorenzuola d'Arda (PC), Italy.

Journal of the Science of Food and Agriculture
|July 3, 2010
PubMed
Summary

Higher steeliness in barley grains is linked to increased C hordeins, which can slow malting modification and affect endosperm permeability. This finding impacts understanding barley quality for malting.

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Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
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Published on: November 8, 2024

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Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
08:15

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

Published on: November 8, 2024

Area of Science:

  • Agricultural Science
  • Plant Biology
  • Food Science

Background:

  • Barley grain endosperm exhibits distinct mealy and steely textures.
  • Steely barley grains often lead to uneven endosperm modification during malting.
  • Understanding the link between steeliness and malting quality is crucial for barley breeding and processing.

Purpose of the Study:

  • To investigate the relationship between barley grain steeliness and endosperm modification during malting.
  • To analyze two barley cv. Scarlett samples with contrasting malting qualities.

Main Methods:

  • Comparative analysis of barley samples with high (77%) and moderate (46%) proportions of steely grains.
  • Assessment of malting modification indicators: beta-glucan degradation, friability, Calcofluor staining, hot water extract (HWE), acrospire growth, and extract viscosity.
  • Evaluation of endosperm permeability to large molecules using fluorescein isothiocyanate-dextran conjugate (FITC-D).

Main Results:

  • The steely barley sample exhibited slower malting modification, lower HWE and acrospire growth, and higher extract viscosity compared to the mealy sample.
  • Endosperm permeation to FITC-D closely followed cell wall modification in the steely sample, but not in the mealy sample.
  • Higher steeliness correlated with increased levels of C hordeins in barley cv. Scarlett.

Conclusions:

  • Increased C hordeins in barley may enhance permeability to large molecules (FITC-D) while retarding malting modification.
  • Steeliness, C hordein levels, and FITC-D permeability appear influenced more by environmental factors than genetics.
  • The study confirms a negative role of C hordeins in malting quality.