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Updated: Jun 11, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
The architecture of RNA polymerase fidelity
1Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA. cdkaplan@tamu.edu
Abstract:
The basis for transcriptional fidelity by RNA polymerase is not understood, but the 'trigger loop', a conserved structural element that is rearranged in the presence of correct substrate nucleotides, is thought to be critical. A study just published in BMC Biology sheds new light on the ways in which the trigger loop may promote selection of correct nucleotide triphosphate substrates. See research article http://www.biomedcentral.com/1741-7007/8/54.
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