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Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary
1Department of Morphology, Physiology and Veterinary Sciences, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno 613 00, Czech Republic. zbysek.sladek@mendelu.cz
Abstract:
This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
Insights
This study reveals that CD14 expression on polymorphonuclear leukocytes (PMN) from heifer mammary glands is linked to apoptosis and necrosis. Resident PMN showed higher levels of CD14, apoptosis, and necrosis compared to inflammatory and blood PMN.
Area of Science:
- Veterinary Immunology
- Cell Biology
- Bovine Health
Background:
- Polymorphonuclear leukocytes (PMN) play a crucial role in mammary gland health.
- CD14 is a cell surface receptor involved in innate immunity.
- Apoptosis and necrosis are key processes in immune cell regulation.
Purpose of the Study:
- To investigate the association between CD14 expression and apoptosis in different PMN populations from heifer mammary glands.
- To determine if CD14 expression correlates with cell death in PMN.
- To compare CD14 expression and apoptosis in resident, inflammatory, and blood PMN.
Main Methods:
- Three-color flow cytometry analysis was used to quantify CD14 expression, apoptosis, and necrosis.
- PMN populations were isolated from heifer mammary glands (resident and inflammatory) and blood.
- In vitro cultivation of PMN populations was performed.
Main Results:
- Fresh resident PMN exhibited significantly higher proportions of CD14+, apoptotic, and necrotic cells compared to inflammatory and blood PMN.
- In vitro cultivation of all PMN types led to a concurrent increase in CD14+, apoptotic, and necrotic cells.
- A positive correlation was observed between CD14+ PMN and both apoptotic and necrotic PMN.
Conclusions:
- CD14 expression in heifer mammary gland PMN is associated with apoptosis and necrosis.
- Resident PMN are more prone to CD14 expression and cell death compared to inflammatory and blood PMN.
- These findings contribute to understanding immune cell dynamics in bovine mastitis.
