Related Experiment Video
Updated: Jun 11, 2026

11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Novel candidate substrates of AMP-activated protein kinase identified in red blood cell lysates
Ramon F Thali1, Roland D Tuerk, Roland Scholz
1Institute of Cell Biology, ETH Zurich, Zurich, Switzerland. ramon.thali@cell.biol.ethz.ch
Biochemical and Biophysical Research Communications
|July 6, 2010
Abstract:
AMPK is a metabolic stress-sensing kinase with important functions for red blood cell (RBC) survival. By using a proteomic approach, we identified putative AMPK targets in hemoglobin-depleted lysates of RBC, including metabolic enzymes, cytoskeletal proteins and enzymes involved in the oxidative stress response. These data tie in with the phenotypic observations of AMPKalpha1-deficient RBC and provide reference for future studies.

