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The effect of mitomycin C in reducing intraarticular adhesion after knee surgery in rabbits
Lianqi Yan1, Yu Sun, Jingcheng Wang
1Department of Orthopedics, Subei People's Hospital, Clinical Medical College of Yangzhou University, Nantong West Road 98, Yangzhou 225001, China.
Abstract:
Mitomycin C (MMC) is known to inhibit fibroblast proliferation through suppressing DNA dependent RNA synthesis. Based on this knowledge, we illustrated the effect of MMC on inhibiting fibroblast collagen synthesis and reducing intraarticular fibrous adhesion in a rabbit model. Forty-eight New Zealand white rabbits were randomly divided into four groups. Approximately 10 x 10 mm squares of the cortical bone was removed from both sides of the femoral condyle, and the underneath cancellous bone was exposed. MMC in various concentrations or saline were then applied to the decorticated areas. The intraarticular adhesions were evaluated after four weeks by macroscopic evaluation, histological evaluation and biochemical analysis of hydroxyproline content. The results demonstrated that MMC could suppress the formation of intraarticular fibrous adhesion in a dose-dependent manner. The intraarticular adhesion score, the hydroxyproline content and the fibroblast number in 0.1mg/ml MMC group were significantly less than those in 0.05 mg/ml MMC group, 0.01 mg/ml MMC group and control group. However, dense adhesions were found in 0.01 mg/ml MMC group and control group. These results indicated that topical application of 0.1mg/ml MMC could reduce intraarticular adhesion through inhibiting fibroblast proliferation in rabbits.
Insights
Mitomycin C (MMC) effectively reduces intraarticular fibrous adhesions in rabbits by inhibiting fibroblast proliferation. This study demonstrates MMC
Area of Science:
- Orthopedics
- Biomedical Engineering
- Pharmacology
Background:
- Mitomycin C (MMC) inhibits fibroblast proliferation by suppressing DNA-dependent RNA synthesis.
- Fibroblast proliferation and collagen synthesis contribute to intraarticular fibrous adhesion formation.
- Effective strategies are needed to prevent post-surgical intraarticular adhesions.
Purpose of the Study:
- To investigate the efficacy of Mitomycin C (MMC) in inhibiting fibroblast collagen synthesis.
- To evaluate the effect of MMC on reducing intraarticular fibrous adhesion in a rabbit model.
- To determine the optimal concentration of MMC for preventing adhesions.
Main Methods:
- Forty-eight New Zealand white rabbits underwent surgical removal of cortical bone from the femoral condyle.
- Varying concentrations of MMC or saline were applied topically to the exposed cancellous bone.
- Intraarticular adhesions were assessed after four weeks using macroscopic, histological, and biochemical (hydroxyproline) analyses.
Main Results:
- MMC demonstrated a dose-dependent suppression of intraarticular fibrous adhesion formation.
- The 0.1 mg/ml MMC group showed significantly lower adhesion scores, hydroxyproline content, and fibroblast numbers compared to other groups.
- Dense adhesions were observed in the 0.01 mg/ml MMC and control groups.
Conclusions:
- Topical application of 0.1 mg/ml Mitomycin C effectively reduces intraarticular adhesions in rabbits.
- MMC's anti-adhesive effect is mediated by the inhibition of fibroblast proliferation.
- This study supports the potential of MMC as a therapeutic agent for preventing joint adhesions.
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