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Cytoplasmic calcium oscillations: a two pool model.

M J Berridge1

  • 1Department of Zoology, University of Cambridge, UK.

Cell Calcium
|February 1, 1991
PubMed
Summary
This summary is machine-generated.

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Calcium oscillations are driven by periodic calcium release, with inositol 1,4,5 trisphosphate (Ins(1,4,5)P3) initiating this process. A two-pool model explains how Ins(1,4,5)P3 and calcium-induced calcium release generate these cellular calcium spikes and waves.

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Physiology

Background:

  • Cytosolic calcium oscillations are common cellular responses to various agonists.
  • Agonists stimulating phosphoinositide metabolism trigger periodic calcium release from intracellular stores.
  • Inositol 1,4,5 trisphosphate (Ins(1,4,5)P3) is a key signaling molecule involved in initiating these calcium oscillations.

Purpose of the Study:

  • To present a two-pool model explaining the mechanism of Ins(1,4,5)P3-induced calcium oscillations.
  • To elucidate the roles of Ins(1,4,5)P3-sensitive and insensitive calcium stores in generating calcium spikes.
  • To explain how calcium waves propagate through cells based on calcium-induced calcium release.

Main Methods:

  • Development of a theoretical two-pool model for calcium dynamics.

Related Experiment Videos

  • Analysis of Ins(1,4,5)P3's role in calcium release and sequestration.
  • Modeling of calcium-induced calcium release (CICR) for wave propagation.

    • Main Results:

    • Ins(1,4,5)P3 initiates oscillations by creating a primer calcium influx (Ca2+p) from sensitive stores and external sources.
    • Primer calcium is sequestered by Ins(1,4,5)P3-insensitive calcium stores (IICS).
    • Filled IICS trigger calcium spikes (Ca2+s) via calcium-induced calcium release, which can propagate as waves.

    Conclusions:

    • The two-pool model effectively explains Ins(1,4,5)P3-mediated cytosolic calcium oscillations.
    • Calcium-induced calcium release is a critical mechanism for generating calcium spikes and waves.
    • The model provides a framework for understanding cellular calcium signaling dynamics.