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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Jun 11, 2026

Purification of Mitochondria from Yeast Cells

Purification of Mitochondria from Yeast Cells

Published on: August 24, 2009

Method to purify mitochondrial DNA directly from yeast total DNA.

Jingwen Zhou¹, Liming Liu, Jian Chen

¹State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

|July 6, 2010

Summary

This study presents a simple enzymatic method to isolate pure mitochondrial DNA (mtDNA) from yeast. The technique effectively removes linear DNA, preserving intact mtDNA for downstream applications like PCR and RFLP analysis.

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Purification of Mitochondria from Yeast Cells

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Published on: May 2, 2025

Area of Science:

Molecular Biology
Yeast Genetics
Biochemistry

Background:

Yeast DNA purification typically yields both nuclear and mitochondrial DNA (mtDNA).
Isolating pure mtDNA often requires complex and time-consuming methods.
Linear DNA contaminants can interfere with downstream molecular analyses of mtDNA.

Purpose of the Study:

To develop a simple enzymatic method for obtaining pure and intact mtDNA from yeast.
To eliminate the need for ultracentrifugation or fractional precipitation in mtDNA isolation.
To provide a method compatible with various yeast species for mtDNA preparation.

Main Methods:

Utilized a combination of lambda exonuclease and RecJ(f) enzymes.
Applied the enzymatic treatment to total DNA isolated from yeast cells.
Assessed mtDNA purity and integrity using PCR amplification and RFLP analysis.

Main Results:

The enzymatic method efficiently removed linear DNA from total yeast DNA.
Mitochondrial DNA (mtDNA) remained intact and pure after enzymatic treatment.
The method was successfully validated in multiple yeast species, including Candida (Torulopsis) glabrata, Saccharomyces cerevisiae, Candida utilis, Pichia pastoris, and Yarrowia lypolytica.

Conclusions:

The described enzymatic approach offers a straightforward and effective way to isolate pure mtDNA.
This method simplifies mtDNA preparation for applications such as PCR amplification and RFLP analysis.
The technique is versatile and applicable across a range of industrially relevant yeast species.