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Related Experiment Videos

Decrease of internal free calcium and human sperm movement.

C Serres1, D Feneux, B Berthon

  • 1Laboratoire de Biologie de la Reproduction et du Développement, Histologie, Embryologie, Cytogénétique, Centre Hospitalier, Kremlin-Bicêtre, France.

Cell Motility and the Cytoskeleton
|January 1, 1991
PubMed
Summary
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Calcium significantly impacts human sperm motility by influencing flagellar movement. Lowering intracellular calcium reduces sperm

Area of Science:

  • Spermatozoa motility
  • Cellular calcium signaling
  • Human reproductive biology

Background:

  • Calcium ions (Ca++) are crucial for various cellular processes, including sperm function.
  • Understanding the precise role of calcium in human sperm motility is essential for reproductive health research.

Purpose of the Study:

  • To elucidate the effects of calcium on human spermatozoa movement.
  • To investigate the relationship between intracellular calcium concentration and sperm motility parameters.

Main Methods:

  • Human spermatozoa were selected using swim-up migration.
  • Intracellular calcium concentration ((Ca++)i) was measured using the fluorescent indicator Quin-2.
  • Sperm movement was analyzed using videomicrography and an automatic analyzer (Hamilton Thorn).

Related Experiment Videos

  • Cells were incubated in BWW medium with or without calcium and varying EGTA concentrations to manipulate (Ca++)i.

    • Main Results:

    • Decreased intracellular calcium concentration ((Ca++)i) was associated with a reduced mean amplitude of lateral head displacement (ALH).
    • Lowering external free calcium by adding EGTA decreased the percentage of spermatozoa with ALH ≥ 6 microns.
    • Spermatozoa with higher ALH exhibited specific flagellar characteristics (proximal tail curvature, low wave velocity/beat frequency) indicating high beat efficiency.
    • This movement pattern disappeared when intracellular calcium was lowered.

    Conclusions:

    • Calcium plays a complex role in regulating the relationship between flagellar curvature and wave propagation in human spermatozoa.
    • Human spermatozoa can modulate their movement in response to alterations in intracellular calcium levels.
    • Calcium is confirmed to play a role in controlling flagellar movement in intact sperm cells.