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Updated: Jun 11, 2026

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Published on: January 17, 2014
Rapid enumeration of respiratory active mycobacteria with fluorescent double staining
Tomoaki Ichijo1, Yoko Izumi, Nobuyasu Yamaguchi
1Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Journal of Microbiological Methods
|July 6, 2010
Abstract:
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach.
