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Updated: Jun 11, 2026
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
Role of brominated diphenly ether-209 in the differentiation of neural stem cells in vitro
Chunfang Zhang1, Xianbao Liu, Dunjin Chen
1The Third Affiliated Hospital of Guangzhou Medical College, The Medical Center for Critical Pregnant Women in Guangzhou, Guangzhou, China.
Abstract:
Brominated diphenly ether-209 (BDE-209, decaBDE) is among the most common flame retardants. In a previous study, it was confirmed that exposure to BDE-209 can decrease learning and memory in mice. However, it is still unknown whether BDE-209 has an effect on cultured neural stem cells (NSCs). To analyse the role of BDE-209 in the differentiation of neural stem cells, NSCs obtained from neonatal rats were cultured as neurospheres in DMEM/F12 medium that contained different concentrations of BDE-209 for 7 days. BDE-209 was found to inhibit neurite outgrowth and the differentiation of NSCs into neurons in a concentration-dependent manner. BDE-209 also enhanced the ratio of differentiation of NSCs into glial cells.
