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The interface between transcription and mRNP export: from THO to THSC/TREX-2
Ana G Rondón1, Sonia Jimeno, Andrés Aguilera
1Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Universidad de Sevilla-CSIC, Av. Americo Vespucio s/n, 41092 Sevilla, Spain.
Biochimica Et Biophysica Acta
|July 6, 2010
Summary
Messenger ribonucleoprotein (mRNP) formation is crucial for eukaryotic gene expression, impacting mRNA processing, export, and transcription. Key factors like THO and THSC/TREX-2 regulate this process and genome stability in Saccharomyces cerevisiae.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Eukaryotic gene expression is a complex, multi-step process from transcription to protein modification.
- Nascent pre-messenger RNA (pre-mRNA) is packaged into messenger ribonucleoprotein (mRNP) particles immediately after transcription by RNA polymerase II (RNAPII).
- Optimal mRNP configuration is essential for pre-mRNA processing, mRNA export, mRNA integrity, and transcription elongation efficiency.
Purpose of the Study:
- To review current knowledge on the role of specific factors in the interface between transcription and mRNA export.
- To highlight the function of THO and THSC/TREX-2 complexes in Saccharomyces cerevisiae.
- To discuss the impact of these factors on transcription-dependent genome instability.
Main Methods:
- Literature review of studies on gene expression, mRNP formation, and mRNA export in Saccharomyces cerevisiae.
- Analysis of the interplay between transcription and mRNP biogenesis.
- Examination of the role of conserved factors, including THO and THSC/TREX-2.
Main Results:
- The formation of mRNPs is tightly coupled with transcription elongation by RNAPII.
- Factors such as THO and THSC/TREX-2 are critical for coordinating transcription, mRNP assembly, and mRNA export.
- These factors also play a significant role in maintaining genome stability, particularly in response to transcription-related stress.
Conclusions:
- The interplay between transcription and mRNP formation is a dynamic and bidirectional process.
- Conserved factors are essential for linking gene expression events from transcription to mRNA export.
- Dysregulation of these factors can lead to both mRNA processing defects and genome instability.
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