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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Source And Potency Of Stem Cells

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Related Experiment Video

Updated: Jun 11, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells.

Ross A Kopher¹, Vesselin R Penchev, Mohammad S Islam

¹Department of Medicine and Stem Cell Institute, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN 55455, USA.

|July 6, 2010

Summary

Human embryonic stem cells (hESCs) can generate mesenchymal stem/stromal cell (MSC) progenitors. These hESC-derived MSCs differentiate and exhibit distinct gene expression, offering new avenues for regenerative therapies.

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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

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Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Published on: October 8, 2009

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Last Updated: Jun 11, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Published on: October 8, 2009

Area of Science:

Stem cell biology
Regenerative medicine
Developmental biology

Background:

Mesenchymal stem/stromal cells (MSCs) are used in therapies, but their developmental origins and precursor cells are not well understood.
Human embryonic stem cells (hESCs) can differentiate into mesodermal cells, including hemato-endothelial cells.
Previous research identified hESC-derived CD73(+) cells with MSC-like properties.

Purpose of the Study:

To identify and characterize MSC progenitor cells derived from hESCs.
To investigate the differentiation potential of hESC-derived MSC progenitors.
To compare the gene expression profile of hESC-derived MSCs with bone marrow-derived MSCs.

Main Methods:

Isolation and characterization of CD34(+)CD73(-) cells from hESC cultures.
Assessment of differentiation capacity into adipocytes, osteoblasts, and chondrocytes.
Gene array analysis to compare gene expression between hESC-derived MSCs and bone marrow-derived MSCs.

Main Results:

hESC-derived CD34(+)CD73(-) cells function as MSC progenitors.
These progenitor cells successfully differentiated into adipocytes, osteoblasts, and chondrocytes.
hESC-derived MSCs displayed significantly different gene expression compared to bone marrow-derived MSCs, with higher expression of pluripotent, multipotent, and endothelial cell-associated genes.

Conclusions:

Functional MSCs can be generated from hESC-derived CD34(+)CD73(-) progenitor cells.
This finding enhances the understanding of MSC developmental pathways.
Utilizing hESC-derived MSCs offers potential for novel regenerative therapies.