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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Updated: Jun 11, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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Pre-embedding nanogold silver and gold intensification.

Akitsugu Yamamoto1, Ryuichi Masaki

  • 1Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|July 6, 2010
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Summary

Pre-embedding nanogold intensification methods enhance immunolabeling sensitivity and resolution for electron microscopy. Silver intensification excels in labeling, while gold intensification offers superior ultrastructural preservation in cultured cells.

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Area of Science:

  • Cell biology
  • Microscopy techniques
  • Immunohistochemistry

Background:

  • Pre-embedding immunolabeling is crucial for ultrastructural analysis.
  • Nanogold technology offers high sensitivity and resolution in microscopy.
  • Simultaneous light and electron microscopy requires optimized labeling methods.

Purpose of the Study:

  • To introduce pre-embedding nanogold silver and gold intensification methods.
  • To evaluate these methods for cultured cells.
  • To compare the efficacy of silver and gold intensification for immunolabeling and ultrastructural preservation.

Main Methods:

  • Immunoreactions using nanogold-labeled antibodies.
  • Pre-embedding intensification of nanogold particles (silver and gold).
  • Embedding and ultrathin sectioning for electron microscopy.
  • Simultaneous light and electron microscopy observation.

Main Results:

  • Both silver and gold intensification methods provide high sensitivity and good resolution.
  • Silver intensification generally yields superior immunolabeling.
  • Gold intensification offers better ultrastructural preservation.
  • Methods are suitable for 3D immunoelectron microscopy.

Conclusions:

  • Pre-embedding nanogold silver and gold intensification are effective for immunolabeling cultured cells.
  • Choice between silver and gold depends on whether labeling or ultrastructure is prioritized.
  • These techniques facilitate correlative light and electron microscopy and 3D analyses.