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Updated: Jun 11, 2026

Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
Published on: June 22, 2012
Charge and size dependence of liposome diffusion in semidilute biopolymer solutions
Laura Rusu1, Dirk Lumma, Joachim O Rädler
1Ludwig-Maximilians-Universität, Fakultät für Physik und Center for NanoScience, Geschwister-Scholl-Platz 1, D-80539 München, Germany.
Abstract:
The diffusion of liposomes and PL/DNA complexes in mucin and collagen solutions, considered to model 'in vivo' colloidal gene delivery vector transport, is studied with FCS. The diffusion of defined liposomes is investigated as a function of particle size, surface charge, and the deviation from the Stokes-Einstein behavior. In all cases the self-diffusion coefficient decreases exponentially with polymer concentration. The same surface charge dependence of diffusion is observed in mucin for PL/DNA complexes with either positive or negative excess charge. Incubation of positively-charged PL/DNA complexes in a natural lung surfactant lipid increases the diffusion coefficients to almost the same as for the negatively-charged PL/DNA complexes.
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