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Updated: Jun 11, 2026
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
Published on: February 18, 2022
Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles
Jeong-Heui Choi1, Tae Woong Na, Md Iqbal Rouf Mamun
1Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, Republic of Korea.
Abstract:
In this study, a method for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC-FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid-0.2 m MgCl(2) in water and acetonitrile as an extraction solvent, and n-hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents.
