Related Experiment Video
Updated: Jun 11, 2026
Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Published on: April 26, 2019
Chromogranin A as a possible tool in the diagnosis of irritable bowel syndrome
Magdy El-Salhy1, Bjarne Lomholt-Beck, Trygve Hausken
1Section for Gastroenterology, Department of Medicine, Stord Helse-Fonna Hospital, Stord, Norway. magdy.el-salhy@helse-fonna.no
Objective:
Serum chromogranin A (CgA) levels have been reported to be normal, reduced or elevated in irritable bowel syndrome (IBS) patients. The aim of the present study was to establish a possible abnormality in CgA plasma level and in intestinal CgA cell density in IBS patients and to evaluate the outcome for the diagnosis of IBS.
Material And Methods:
Forty-one patients with irritable bowel syndrome according to Rome Criteria III (39 females and 2 males; average age 35 years) and 59 healthy controls (37 females and 22 males; average age 45.5 years) were included in the study. Duodenal and colonic biopsies were obtained from all IBS patients. Forty-two of these healthy subjects underwent gastroscopy with duodenal biopsies. The remaining 17 subjects underwent colonoscopy with biopsies. The biopsies were immunostained with avidin-biotin-complex method for CgA cells. The cell densities were quantified by computerized image analysis. CgA plasma level was determined with ELISA technique.
Results:
The density of CgA cells in both the duodenum and colon was reduced in patients with IBS. CgA cell density in the left colon was, however, unaffected in patients with IBS-constipation. There was no difference in the plasma level of CgA between patients with IBS and controls.
Conclusion:
The reduced density of intestinal CgA cells should be considered as a reduction in the total amount of intestinal endocrine cells. Which endocrine cell type is affected remains to investigate. This reduction may offer a histopathological test for the diagnosis of IBS. It is doubtful that the blood level of CgA has any clinical impact in IBS.
More Related Videos
10:37Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
08:02Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
Published on: March 12, 2020
Related Concept Videos
01:30Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation
Irritable Bowel Syndrome (IBS) is classified into subtypes based on the predominant bowel habits as determined by the Bristol Stool Form Scale (BSFS). The subtypes are:
01:23Irritable Bowel Syndrome
01:17Irritable Bowel Syndrome I: Introduction
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
01:23Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the colonic...
01:30Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by transmural...