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Updated: Jun 11, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
David-Olivier D Azulay1, Hendrik Neubert, Mireia Fernández Ocaña
1Pfizer Global Research and Development, Sandwich, UK. david-olivier.azulay@pfizer.com
Background:
OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality.
Results:
We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions.
Conclusions:
A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.
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