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Electrophoretic characterization of human, equine and bovine transferrins
R C Penhallow1, A B Mason, R C Woodworth
1Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
Abstract:
1. Human, bovine and equine transferrins have been characterized with respect to mol. wt, and behavior on urea-polyacrylamide gels, and isoelectric focussing gels. 2. As shown by SDS-polyacrylamide gel electrophoresis human transferrin has one major polypeptide whereas both bovine and equine transferrins have two polypeptides. 3. The transferrins show multiple banded patterns on urea-polyacrylamide and isoelectric focussing gels, particularly when iron saturated. The various forms are not resolved by neuraminidase treatment.