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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jun 11, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Surface plasmon resonance immunosensor for bacteria detection.

H Baccar1, M B Mejri, I Hafaiedh

  • 1Nanotechnology Laboratory, INSAT, Centre Urbain Nord, Charguia, Tunisia.

Talanta
|July 7, 2010
PubMed
Summary
This summary is machine-generated.

Two novel bacteria biosensors utilizing surface plasmon resonance (SPR) were developed for detecting Escherichia coli (E. coli). The biosensors achieved detection limits of 10^4 and 10^3 colony-forming units per milliliter (cfu mL-1).

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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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Last Updated: Jun 11, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

Area of Science:

  • Biosensor development
  • Surface Plasmon Resonance (SPR) technology
  • Bacteriology

Background:

  • Development of sensitive and specific biosensors is crucial for bacterial detection.
  • Surface Plasmon Resonance (SPR) offers a label-free method for real-time monitoring of biomolecular interactions.

Purpose of the Study:

  • To develop and evaluate two distinct bacteria biosensors using SPR.
  • To assess the detection limits and specificity of the developed biosensors for Escherichia coli (E. coli).

Main Methods:

  • Fabrication of two SPR-based biosensors: one with a functionalized gold substrate and another with immobilized gold nanoparticles.
  • Functionalization of gold substrates using acid-thiol self-assembled monolayers and immobilization of gold nanoparticles.
  • Immobilization of polyclonal anti-Escherichia coli antibodies for specific bacterial capture.

Main Results:

  • Achieved detection limits of 10^4 cfu mL(-1) for the functionalized gold substrate biosensor and 10^3 cfu mL(-1) for the gold nanoparticle biosensor.
  • Demonstrated specific detection of E. coli against non-specific bacteria (Lactobacillus).
  • The biosensors could detect refractive index variations below 5x10(-3) caused by bacterial adsorption.

Conclusions:

  • The developed SPR-based biosensors show promise for sensitive and specific detection of E. coli.
  • The gold nanoparticle-based biosensor offers enhanced sensitivity compared to the functionalized gold substrate.
  • Further modeling provided insights into the refractive index changes within the biosensor layers.